Sequence-Based Typing of flaB Is a More Stable Screening Tool Than Typing of flaA for Monitoring of Campylobacter Populations
Open Access
- 1 October 2004
- journal article
- research article
- Published by American Society for Microbiology in Journal of Clinical Microbiology
- Vol. 42 (10) , 4840-4842
- https://doi.org/10.1128/jcm.42.10.4840-4842.2004
Abstract
Different typing schemes for Campylobacter spp. were evaluated with 70 outbreak and sporadic isolates. The discriminatory indexes were 0.944 (by pulsed-field gel electrophoresis), 0.920 (by genotyping of the flagellin A gene), 0.902 (by genotyping of flaB ), and 0.886 (by multilocus sequence typing). Cross-classification gave 94.77 or 95.82% (PFGE- flaA or PFGE- flaB ) concordance. flaA was overdiscriminatory in three cases, most probably due to intragenomic recombination.Keywords
This publication has 15 references indexed in Scilit:
- Typing of Methicillin-Resistant Staphylococcus aureus in a University Hospital Setting by Using Novel Software for spa Repeat Determination and Database ManagementJournal of Clinical Microbiology, 2003
- Utility of Multilocus Sequence Typing as an Epidemiological Tool for Investigation of Outbreaks of Gastroenteritis Caused by Campylobacter jejuniJournal of Clinical Microbiology, 2003
- Determining Confidence Intervals When Measuring Genetic Diversity and the Discriminatory Abilities of Typing Methods for MicroorganismsJournal of Clinical Microbiology, 2001
- Rapid Pulsed-Field Gel Electrophoresis Protocol for Subtyping of Campylobacter jejuniJournal of Clinical Microbiology, 2001
- Multilocus Sequence Typing System for Campylobacter jejuniJournal of Clinical Microbiology, 2001
- Rohmilch als Ursache lebensmittelbedingter Campylobacter-InfektionenBundesgesundheitsblatt - Gesundheitsforschung - Gesundheitsschutz, 2000
- Genotyping of Campylobacter sppApplied and Environmental Microbiology, 2000
- Consensus guidelines for appropriate use and evaluation of microbial epidemiologic typing systemsClinical Microbiology & Infection, 1996
- Variation of the flagellin gene locus of Campylobacter jejuni by recombination and horizontal gene transferMicrobiology, 1995
- Significance of Duplicated Flagellin Genes in CampylobacterJournal of Molecular Biology, 1993