EFFECTS OF DIFFERENT AFTER-RIPENING TREATMENTS ON GERMINATION AND ENDOGENOUS GROWTH INHIBITORS IN ROSA RUGOSA

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Abstract
The concentration of growth and germination inhibitors in achenes from seedlings of R. rugosa Thunberg is reduced through leaching. Leaching of achenes for 24 hr before stratification increased the germination but the effect of leaching was not always significant. The presence of inhibitors was indicated between Rf 0.3–0.4 and Rf 0.7–0.9 by the retarding effect on radicle elongation of cress seeds (Lepidium sativum L. cult Moss Curled) when extracts of achenes were developed on ascending paper chromatograms (isopropanol:ammonia:water, 10:1:1, v/v). Changed concentrations of the inhibitor between Rf 0.7 and 0.9 from different after-ripening treatments did affect the germination of the achenes but the changed concentrations of the inhibitor between Rf 0.3 and 0.4 did not. Presumably, the inhibitor between Rf 0.7 and 0.9 was abscisic acid. Warm–cold treatments promoted germination more effectively than cold treatments. The most effective treatment was 4 weeks at 20 C and 8 weeks at 4 C. Both cold and warm–cold after-ripening seemed to promote germination through a reduction of growth inhibitors. Inhibitors do not control the germination of rose achenes exclusively because: (a) leaching reduced the concentration of inhibitors but it did not induce germination without after-ripening treatments; (b) partial after-ripening for 4 weeks at 20 C also reduced the concentration of inhibitors but it did not induce germination unless it was followed by an after-ripening period of 4 C; (c) after-ripened achenes germinated in spite of inhibitors.

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