The molecular cloning of the squid (Loligo forbesi) visual Gq-α subunit and its expression in Saccharomyces cerevisiae
- 1 June 1993
- journal article
- Published by Portland Press Ltd. in Biochemical Journal
- Vol. 292 (2) , 333-341
- https://doi.org/10.1042/bj2920333
Abstract
The sequence of the alpha-subunit of the major G-protein from the squid (Loligo forbesi) retina was predicted from its cDNA to be a member of the Gq subclass. The abundance of the squid Gq-alpha in the squid photoreceptor membranes suggests that the protein functions in phototransduction; the sequence of this G-protein is consistent with it mediating the light-dependent activation of a phospholipase C. The squid G-alpha was expressed in the yeast Saccharomyces cerevisiae, where it was unable to replace the function of GPA1, the yeast G-alpha homologue that regulates the mating response, suggesting that Gq-alpha was unable to interact with the endogenous G-beta gamma (STE4-STE18).Keywords
This publication has 42 references indexed in Scilit:
- Light-induced GTPase activity and GTP[γS] binding in squid retinal photoreceptorsVisual Neuroscience, 1991
- A Gβ protein in the drosophila compound eye is different from that in the brainNeuron, 1991
- Widespread distribution of Gqα/Gllα detected immunologically by an antipeptide antiserum directed against the predicted C‐terminal decapeptideFEBS Letters, 1991
- Regulation of Polyphosphoinositide-specific Phospholipase C Activity by Purified G qScience, 1991
- New yeast-Escherichia coli shuttle vectors constructed with in vitro mutagenized yeast genes lacking six-base pair restriction sitesGene, 1988
- Molecular cloning and DNA sequence analysis of the human guanine nucleotide-binding protein GoαBiochemical and Biophysical Research Communications, 1988
- The yeast SCG1 gene: A Gα-like protein implicated in the a- and α-factor response pathwayCell, 1987
- Human Gi protein α‐subunit: deduction of amino acid structure from a cloned cDNAFEBS Letters, 1987
- Identification by molecular cloning of two forms of the α‐subunit of the human liver stimulatory (Gs) regulatory component of adenylyl cyclaseFEBS Letters, 1986
- Light-regulated biochemical events in invertebrate photoreceptors. 1. Light-activated guanosine triphosphatase, guanine nucleotide binding, and cholera toxin-catalyzed labeling of squid photoreceptor membranesBiochemistry, 1984