USE OF UNSCHEDULED DNA-SYNTHESIS IN FRESHLY ISOLATED HUMAN INTESTINAL MUCOSAL CELLS FOR CARCINOGEN DETECTION
- 1 January 1980
- journal article
- research article
- Vol. 40 (9) , 3155-3157
Abstract
Mucosal cells freshly isolated from human intestine with pronase retain the capacity to undergo DNA repair synthesis (unscheduled DNA synthesis) during a 2-h exposure to the carcinogen, N-methyl-N''-nitro-N-nitrosoguanidine, and the procarcinogen, aflatoxin B1. This procedure may provide a highly relevant and convenient test system for the detection of cell-specific, direct-acting and activation-dependent chemical carcinogens. The use of whole-cell preparations in such in vitro studies may be of additional significance in view of growing evidence for artifactual metabolism by subcellular fractions.This publication has 8 references indexed in Scilit:
- Epithelioid cell cultures from rat small intestine. Characterization by morphologic and immunologic criteria.The Journal of cell biology, 1979
- Comparison of the metabolic profiles of benzo[a]pyrene obtained from primary cell cultures and subcellular fractions derived from normal and methylcholanthrene-induced rat liverCancer Letters, 1978
- Further improvements in the hepatocyte primary culture DNA repair test for carcinogens: detection of carcinogenic biphenyl derivativesCancer Letters, 1978
- TESTING OF KNOWN CARCINOGENS AND NONCARCINOGENS FOR THEIR ABILITY TO INDUCE UNSCHEDULED DNA-SYNTHESIS IN HELA-CELLS1978
- DETECTION OF CHEMICAL CARCINOGENS BY UNSCHEDULED DNA-SYNTHESIS IN RAT-LIVER PRIMARY-CELL CULTURES1977
- Aflatoxin—Human Colon Carcinogenesis?Annals of Internal Medicine, 1976
- A simple and rapid method to prepare dispersions of columnar epithelial cells from the rat intestineJournal of Ultrastructure Research, 1968
- GEOGRAPHICAL PATHOLOGY OF PRIMARY LIVER CANCER1963