Cell Inclusions and Endospore Formation in Bacillus mycoides

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Abstract
Cells of B. mycoides, at time of spore formation, contain refractive granules regarded as cell in clusions of fat-like nature. Spore formation is not due to fusion of these granules, to growth of a single small refractive granule, or to cell shrinkage with extrusion of water. The spore arises by differentiation of the contents of a polar primordium set off from the remainder of the cell by a membrane. The vorspore consists of anendosporium or definitive body of the spore and a surrounding layer of hyaline cytoplasm, the exosporium. The endosporium develops a true spore wall, while the exosporium is gradually reduced to remnants or completely disappears. The refractive granules disappear during spore ripening. They probably contribute material for later stages of spore development and for metabolic processes of the parent cell. Endospores are regarded as asexual reproductive bodies analogous to spores of fungi.

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