1H‐NMR study of the interaction of aminopyrine with purified rat liver microsomal cytochrome P‐450
- 25 February 1985
- journal article
- Published by Wiley in FEBS Letters
- Vol. 181 (2) , 295-299
- https://doi.org/10.1016/0014-5793(85)80279-9
Abstract
Longitudinal relaxation (T 1) measurements for all lines (N(CH3)2, N(CH3), (C(CH3), phenyl) in the aminopyrine 1H-NMR spectrum were used to study the interaction of aminopyrine with purified microsomal cytochrome P-450 from livers of phenobarbital-treated rats. The paramagnetic contribution to the observed t 1 −1 values was determined from its dependence on aminopyrine concentration. The Solomon-Bloembergen equation was used to calculate between Fe3+ and aminopyrine distances in the enzyme-substrate complex. For all protons these distances are about 8 Å.Keywords
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