Bone Cells: Biochemical and Biological Studies after Enzymatic Isolation
- 11 December 1964
- journal article
- research article
- Published by American Association for the Advancement of Science (AAAS) in Science
- Vol. 146 (3650) , 1476-1477
- https://doi.org/10.1126/science.146.3650.1476
Abstract
Short-term incubation of rat calvaria in buffered crude collagenase permitted the isolation of morphologically intact cells that absorb vital dyes, contain alkaline phosphatase, and multiply in tissue culture. Freshly harvested cells were similar to whole bone segments in aerobic glucose metabolism.Keywords
This publication has 9 references indexed in Scilit:
- Metabolism of Isolated Fat CellsJournal of Biological Chemistry, 1964
- MINERALIZATION OF EMIGRATING BONE CELLS IN TISSUE CULTURE1964
- METABOLISM OF ISOLATED FAT CELLS .I. EFFECTS OF HORMONES ON GLUCOSE METABOLISM + LIPOLYSIS1964
- Aerobic Metabolism of Glucose by BoneJournal of Biological Chemistry, 1962
- A simple efficient liquid scintillator for counting aqueous solutions in a liquid scintillation counterAnalytical Biochemistry, 1960
- The Cytodifferentiation of Osteoblasts in Tissue CultureJournal of Bone and Joint Surgery, 1960
- Mammalian Cell Cultures for Study of Influenza Virus. I. Preparation of Monolayer Cultures with Collagenase.Experimental Biology and Medicine, 1959
- Cultivation and behavior in vitro of the normal mammary epithelium of the adult mouse *1II. Observations on the secretory activityExperimental Cell Research, 1957
- A Histochemical Procedure for Localizing and Evaluating Leukocyte Alkaline Phosphatase Activity in Smears of Blood and MarrowBlood, 1955