The Effect of N Acetyl D-Galactosamine and Other Sugars on the Mitogenic Activity and Attachment of PHA to Tonsil Cells
Open Access
- 1 June 1968
- journal article
- research article
- Published by American Society of Hematology in Blood
- Vol. 31 (6) , 747-757
- https://doi.org/10.1182/blood.v31.6.747.747
Abstract
The effect of several simple sugars on the mitogenic and binding properties of PHA has been investigated. N acetyl D-galactosamine in concentration of 3-10 mg./ml. inhibited the incorporation of uridine-2-C14 and thymidine-2-C14 into the acid precipitable fraction of human tonsil cells stimulated by phytohemagglutinin. No inhibition, or a significantly lesser degree of inhibition was observed with N acetyl D-glucosamine or glucose. The binding of I131 labeled PHA to cells was decreased by NAGAL or EDTA. The EDTA inhibition, unlike the NAGAL inhibition was overcome by magnesium.This publication has 3 references indexed in Scilit:
- Phytohemagglutinin: Inhibition of the Agglutinating Activity by N -Acetyl-D-GalactosamineScience, 1966
- High Specific Activity Labeling of Protein with I131 by the Iodine Monochloride Method.Experimental Biology and Medicine, 1966
- PHYTOHEMAGGLUTININ - AN INITIATOR OF MITOSIS IN CULTURES OF NORMAL HUMAN LEUKOCYTES1960