Gas Chromatographic/Thin-Layer Chromatographic Analysis of Acetylated Codeine and Morphine in Urine
Open Access
- 1 September 1975
- journal article
- research article
- Published by Oxford University Press (OUP) in Clinical Chemistry
- Vol. 21 (10) , 1486-1489
- https://doi.org/10.1093/clinchem/21.10.1486
Abstract
This procedure positively identifies codeine and morphine in urine. Urine samples are hydrolyzed and extracted with organic solvent, and the extracts are evaporated and acetylated. The presence of codeine and morphine is ascertained by gas chromatography (3% OV-25 and 3% Poly-A 103 columns) and confirmed by thin-layer chromatography (system: ethyl acetate/acetone/concd ammonium hydroxide, 100/10/4.5 by vol; reagent: iodoplatinate). As little as 0.5 mg each of codeine and morphine per liter, in free and conjugated forms, is detectable by this method.Keywords
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