Downregulation of Gαq-11protein expression in guinea pig antral and colonic circular muscle during pregnancy

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Abstract
Pregnancy has an inhibitory effect on motility of the gastrointestinal tract. The present study was designed to examine the mechanisms responsible for antral and colonic hypomotility in pregnant guinea pigs. Circular smooth muscle cells from the antrum and left colon were isolated by enzymatic digestion with collagenase from pregnant and nonpregnant guinea pigs. Contractile responses to agonists were expressed as percent shortening from resting cell length. The function of G proteins in antral and colonic circular smooth muscle was assessed by [35S]guanosine 5′- O-(3-thiotriphosphate) (GTPγS) binding induced by CCK-8 and G protein quantitation. The contraction of antral and colonic circular smooth muscle from pregnant guinea pigs was reduced in response to CCK-8 and to GTPγS but was normal in response to KCl andd- myo-inositol 1,4,5-trisphosphate compared with nonpregnant animals. The stimulation of [35S]GTPγS binding to Gαq-11induced by 1 μM CCK-8 was significantly lower in antral and colonic circular smooth muscle from pregnant guinea pigs than that in controls. Furthermore, Western blot analysis showed a decreased Gαq-11and an increased Gsα protein content in both tissues during pregnancy. It is concluded that pregnancy appears to impair gastrointestinal circular smooth muscle contractility by downregulating G proteins such as Gαq-11protein, which mediates muscle contraction, and upregulating Gsα protein, which mediates muscle relaxation.