Fragments of the internal transcribed spacer 1 of pre-rRNA accumulate in Saccharomyces cerevisiae lacking 5'----3' exoribonuclease 1
- 1 November 1991
- journal article
- Published by American Society for Microbiology in Journal of Bacteriology
- Vol. 173 (21) , 7024-7028
- https://doi.org/10.1128/jb.173.21.7024-7028.1991
Abstract
The portion of the internal transcribed spacer 1 found on 20S pre-rRNA accumulates in Saccharomyces cerevisiae lacking 5'----3' exoribonuclease 1, showing that an endonucleolytic cleavage at the 3' terminus of 18S rRNA is involved in the 20S pre-rRNA to 18S mature rRNA conversion. Smaller fragments of the spacer sequence are also found. The exoribonuclease may be involved as a cytoplasmic RNase in the hydrolysis of the spacer.Keywords
This publication has 21 references indexed in Scilit:
- Disruption of the gene XRN1, coding for a 5′→3′ exoribonuclease, restricts yeast cell growthPublished by Elsevier ,2002
- [30] Labeling of RNA and phosphoproteins in Saccharomyces cerevisiaePublished by Elsevier ,1991
- [9] Isolation and characterization of yeast ribosomal RNA precursors and preribosomesPublished by Elsevier ,1989
- Purification and characterization of an activity from Saccharomyces cerevisiae that catalyzes homologous pairing and strand exchange.Proceedings of the National Academy of Sciences, 1987
- A 5′ → 3′ exoribonuclease of Saccharomyces cerevisiae: Size and novel substrate specificityArchives of Biochemistry and Biophysics, 1987
- Lariat structures are in vivo intermediates in yeast pre-mRNA splicingCell, 1984
- Isolation and properties of a single-strand 5' .fwdarw. 3' exoribonuclease from Ehrlich ascites tumor cell nucleoliBiochemistry, 1984
- Genomic sequencing.Proceedings of the National Academy of Sciences, 1984
- Some characteristics of processing sites in ribosomal precursor RNA of yeastNucleic Acids Research, 1980
- Terminal Nucleotide Sequences of 17‐S Ribosomal RNA and Its Immediate Precursor 18‐S RNA in YeastEuropean Journal of Biochemistry, 1977