NEUTROPHIL AGGREGATION AND DEGRANULATION - EFFECT OF ARACHIDONIC-ACID

  • 1 January 1979
    • journal article
    • research article
    • Vol. 95  (2) , 433-444
Abstract
In response to aggregating and degranulating stimuli, platelets metabolize endogenous arachidonic acid to bioactive derivatives. These derivatives can stimulate platelets to degranulate and aggregate and, therefore, may be mediators of the platelet response. Because exogenous arachidonic acid also stimulates platelets to degranulate, aggregate and form these mediators, the effect of adding arachidonic acid to purified human neutrophil suspensions was examined. Micromolar concentrations of arachidonic acid stimulated neutrophils to aggregate but not to degranulate. Cytochalasin B, a potentiator of neutrophil responses to chemotactic factors, also potentiated the arachidonic acid-induced aggregation response. 5,8,11,14-Eicosatetraynoic acid, an inhibitor of arachidonic acid metabolism, blocked this response. Aggregation of neutrophils was not stimulated by several fatty acids with structural similarity to arachidonic acid. Metabolic derivatives of arachidonic acid may be active in stimulating certain neutrophil responses. The role of these derivatives in mediating neutrophil responses to various stimuli needs to be examined.

This publication has 60 references indexed in Scilit: