LARGE-SCALE PURIFICATION OF PROTHORACICOTROPIC HORMONE OF THE SILKWORM (BOMBYX-MORI)

  • 1 January 1980
    • journal article
    • research article
    • Vol. 23  (8) , 1053-1060
Abstract
Prothoracicotropic hormone was extracted from .apprx. 2,800,000 silkworm male adult heads and partially purified during an 8 step procedure. Approximately 7 .mu.g of the crude preparation (crude PTTH) shows activity in the debrained Samia pupa test. A 4 step chromatographic purification of the crude PTTH derived from 100,000 Bombyx heads yielded 3.4 mg of purified preparation (highly purified PTTH), of which 7 ng causes adult development in a debrained Samia pupa.

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