Concentration and purification of rubella virus hemagglutinin by hollow fiber ultrafiltration and sucrose density centrifugation

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Abstract
Large volumes of rubella virus were produced in Vero cell monolayers which were grown in the Corbeil-Bellco TM system. Infectious tissue culture fluids were concentrated at least 600 times in less than 4 h by ultrafiltration on hollow fibers with a molecular weight cutoff of 100 000. Recovery of the hemagglutinating activity was 75%. Rubella virus was purified by three successive sucrose density gradient centrifugations using a combination of discontinuous and linear gradients. Specific activity was increased 1000-fold.