Crystalline lysoplasmalogen (lysophosphatidal choline): preparation from heart muscle and action on erythrocytes and spermatozoa

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Abstract
A choline-based lysoplasmalogen has been prepared in crystalline form from an alkaline hydrolysate of the lecithin fraction from ox heart. The ratio phosphorus: choline: fatty aldehyde is 1:1:1. Lysoplasmalogen, hydrogenated lysoplasmalogen and lysolecithin are quantitatively equivalent as lysins for horse erythrocytes. The respiration of ram-sperm suspensions (3 X 108 cells/ml) is inhibited by lysolecithin only at concentrations greater than 1 m[image] The other 2 lyso compounds are effective inhibitors at lower concentrations. Fructolysis in ram semen is also inhibited by lysoplasmalogen. No free fatty aldehyde is formed when lysoplasmalogen is incubated with ram spermatozoa.