Arginine-requiring Strains of Tissue Obtained from Ginkgo Pollen

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Abstract
Sterile stored pollen of G. biloba was plated out aseptically on defined media containing various amino acids. Tissues were obtained from media with arginine. These tissues are considered biochemical mutants selected from large numbers of cultured pollen; they grow rapidly and continuously and are un-differentiated. In the nutrition of the tissues, ammonia, lysine, ornithine, and urea, in that order, partly substitute for arginine. The optimum concentration of arginine is 500-1000 ppm. Proline promotes growth but hydroxyproline is inhibitory; arginine partly reverses the inhibition by hydroxyproline. Canavanine inhibition is reversed by arginine only to a slight extent.