2-DEOXY-GLUCOSE-6-PHOSPHATE UTILIZATION IN THE STUDY OF GLUCOSE-6-PHOSPHATE-DEHYDROGENASE MOSAICISM

  • 1 January 1981
    • journal article
    • research article
    • Vol. 33  (2) , 307-313
Abstract
The electrophoretic difference between normal human glucose-6-phosphate dehydrogenase (G6PD) and 2 common variants (G6PD A and G6PD A-) made the G6PD enzyme system very useful for genetic studies and for investigation on the clonal origin of tumors. This approach has not been possible for another common variant, G6PD Mediterranean, which has a normal electrophoretic pattern. The different utilization of 2-deoxyglucose-6-phosphate (2dG6P), an analog of the normal substrate, by the normal enzyme and the Mediterranean variant, allows a convenient determination of the degree of mosaicism in mononuclear cells from heterozygotes.