From rhizomes of Hemsleya macrosperma, three new saponins of oleanolic acid named hemslosides-Ma1, -Ma2 and -Ma3 were isolated along with dihydrocucurbitacin F, its 25-O-acetate and β-glucosyl oleanolate and their structures were elucidated. From rhizomes of H. chinensis, dihydrocucurbitacin F, its 25-O-acetate, β-glucosyl oleanolate, hemslosides-Ma1 and -Ma3 and chikusetsusaponin-IVa (a saponin from Panax japonicus and other Panax spp.) were also isolated. The close structural relationship of saponins of these planiste those of Panax japonicus (Araliaceae) is noted.