The Threonine‐Sensitive Homoserine Dehydrogenase and Aspartokinase Activities of Escherichia coli K12
- 1 January 1975
- journal article
- Published by Wiley in European Journal of Biochemistry
- Vol. 50 (2) , 425-430
- https://doi.org/10.1111/j.1432-1033.1975.tb09819.x
Abstract
2-Amino-4-oxo-5-chloropentanoic acid inactivates specifically the homoserine dehydrogenase activity of the bifunctional enzyme, aspartokinase I--homoserine dehydrogenase I. The aspartokinase activity remains essentially untouched and retains its threonine sensitivity. The inactivation of the dehydrogenase requires the covalent binding of one equivalent of the analogue per subunit. Alkylation does not affect the tetrameric state of the protein. The alkylating agent, a substrate analogue, meets the qualitative and quantitative requirements of an affinity label.Keywords
This publication has 14 references indexed in Scilit:
- The Threonine‐Sensitive Homoserine Dehydrogenase and Aspartokinase Activities of Escherichia coli K 12European Journal of Biochemistry, 1973
- The Threonine‐Sensitive Homoserine Dehydrogenase and Aspartokinase Activities of Escherichia coli K12European Journal of Biochemistry, 1972
- Revised Structure of Aspartokinase I‐Homoserine Dehydrogenase I of Escherichia coli K12European Journal of Biochemistry, 1972
- Threonine-sensitive aspartokinase-homoserine dehydrogenase complex amino acid compositions, molecular weight, and subunit composition of the complexBiochemistry, 1972
- Threonine-sensitive aspartokinase-homoserine dehydrogenase of Escherichia coli K 12. Reaction with 6-mercapto-9-β-D-ribofuranosylpurine 5'-triphosphateBiochemistry, 1971
- The Threonine-Sensitive Homoserine Dehydrogenase and Aspartokinase Activities of Escherichia coli K 12. Binding of Threonine and of Pyridine Nucleotides: Stoichiometry and Optical EffectsEuropean Journal of Biochemistry, 1969
- Two aspartokinases from Escherichia coli. Nature of the inhibition and molecular changes accompanying reversible inactivationBiochemistry, 1968
- The threonine-sensitive homoserine dehydrogenase and aspartokinase activities of Escherichia coliBiochimica et Biophysica Acta (BBA) - Enzymology and Biological Oxidation, 1966
- Sulfonyl Fluorides as Inhibitors of Esterases. II. Formation and Reactions of Phenylmethanesulfonyl α-Chymotrypsin*Biochemistry, 1964
- A simple efficient liquid scintillator for counting aqueous solutions in a liquid scintillation counterAnalytical Biochemistry, 1960