Über eine biologische Synthese der Pantothensäure. II. Ammonium-Ion als Aktivator

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Abstract
A yeast prepn. is obtained by freezing yeast M in liquid air, and dialyzing the mixture at 30[degree] against phosphate buffer, pH 8, which synthesizes (+ ) pantothenic acid from [beta]-alanine and ([long dash])[alpha]-hydroxy-[beta],[beta]-dimethyl-[gamma]-butyro-lactone at pH 8. The amt. of pantothenic acid formed is detd. with Streptobacterium plantarum. A nutrient soln. is prepd. for yeast M, which contains 37 constituents, including glucose, amino acids, vitamins, inorganic acids, salts, etc. The reaction mixture contains 600-750 mg. of yeast prepn., 0.1 ml. of a 1% soln. of the d,l-lactone and [beta]-alanine in water, 0-4 ml. M/15 phosphate buffer and 0-4 ml. nutrient soln. The reaction is carried out for 24 hrs. at 30[degree]. Without the nutrient soln., the yield of pantothenic acid is 3%, and with the nutrient soln., about 35%. The activating effect of the nutrient soln. is due to the NH4 ion, the opt." conc. of which is 0.1 M per liter in the synthesis mixture. Since substitution of the amide of [alpha],[gamma]-dihydroxy-[beta],[beta]-di-methyl butyric acid for the lactone in the reaction mixture decreases the yield of pantothenic acid, the NH4 ion does not activate the pantothenic acid synthesis by forming the amide.

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