NFκB decoy oligodeoxynucleotides reduce monocyte infiltration in renal allografts
Open Access
- 1 April 2000
- journal article
- research article
- Published by Wiley in The FASEB Journal
- Vol. 14 (5) , 815-822
- https://doi.org/10.1096/fasebj.14.5.815
Abstract
Monocyte influx secondary to ischemia-reperfusion conditions the renal allograft to rejection by presentation of antigens and production of cytokines. Monocyte influx depends on NFicB-dependent transcription of genes encoding adhesion molecules and chemokines. Here we demonstrate that cationic liposomes containing phosphorothioated oligodeoxynucleotides (ODN) with the kB binding site serving as competitive binding decoy, can prevent TNF-α-induced NFκB activity in endothelial cells in vitro. In an allogenic rat kidney transplantation model (BN to LEW), we show that perfusing the renal allograft with this decoy prior to transplantation abolishes nuclear NFκB activity in vivo and inhibits VCAM-1 expression in the donor endothelium (PPPFASEB J. 14, 815–822 (2000)Keywords
Funding Information
- Deutsche Forschungsgemeinschaft
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