Electron-paramagnetic-resonance studies on the molybdenum of nitrate reductase from Escherichia coli K12

1 April 1976

journal article
research article
Published by Portland Press Ltd. in Biochemical Journal

Vol. 155 (1) , 201-203
https://doi.org/10.1042/bj1550201

Abstract

Studies on the respiratory nitrate reductase (EC 1.7.99.4) from Escherichia coli K12 by electron-paramagnetic-resonance spectroscopy indicate that its molybdenum centre is comparable with that in other molybdenum-containing enzymes. Two Mo(V) signals may be observed; one shows interaction of Mo(V) with a proton exchangeable with the solvent and has: A (1H) 0.9-1.2mT; g1 = 1.999; g2=1.985; g3 = 1.964; gav. = 1.983. Molybdenum of both signal-giving species may be reduced with dithionite and reoxidized with nitrate.

This publication has 17 references indexed in Scilit:

A new non-functional form of milk xanthine oxidase containing stable quinquivalent molybdenum
Biochemical Journal, 1976
Studies by electron-paramagnetic-resonance spectroscopy and stopped-flow spectrophotometry on the mechanism of action of turkey liver xanthine dehydrogenase
Biochemical Journal, 1976
Studies by electron-paramagnetic-resonance spectroscopy on the mechanism of action of xanthine dehydrogenase from Veillonella alcalescens
Biochemical Journal, 1976
The purification and properties of formate dehydrogenase and nitrate reductase from Escherichia coli.
Journal of Biological Chemistry, 1975
Synthesis and sideedness of membrane-bound respiratory nitrate reductase (EC1.7.99.4) in Escherichia coli lacking cytochromes.
1975
Purification and Properties of Nitrate Reductase from Escherichia coli K12
Journal of Biological Chemistry, 1974
The Bacterial Nitrate Reductases
European Journal of Biochemistry, 1974
Molybdenum–Thiol Complexes as Models for Molybdenum Bound in Enzymes
Nature, 1966
Nitrate reductase from Pseudomonas aeruginosa
Biochimica et Biophysica Acta, 1961
MOLYBDENUM AND NITRATE REDUCTASE .2. MOLYBDENUM AS A CONSTITUENT OF NITRATE REDUCTASE
1954