ISOLATION AND CHARACTERIZATION OF A GLYCOPROTEIN (JBB5) IN URINE OF A PATIENT WITH CARCINOMA OF COLON

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  • 1 January 1977
    • journal article
    • research article
    • Vol. 37  (3) , 873-878
Abstract
A patient with metastatic carcinoma of the colon excreted 0.5-1.0 g protein daily, about 1/3 of which was in MW class 30,000-60,000. The major component of this class was isolated by gel filtration and ion exchange chromatography. The purified protein, labeled JBB5, contained about 11% sialic acid, 8% hexose and 4% hexosamine. Its MW was between 51,000 and 59,000. It did not react detectably with antisera to any of the recognized normal human plasma proteins. A specific antiserum to JBB5 was raised in the rabbit. Urine from 4% of subjects with nonneoplastic illnesses reacted in double immunodiffusion with anti-JBB5. Thirty-three percent positive reactions were obtained with urines from patients with advanced neoplastic disease, the percentage varying from 64% in metastatic cancer of the pancreas to 15% in chronic lymphocytic leukemia.

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