Enhancement of in-vitro Translation of Eukaryotic RNAs by Cyclic AMP

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Abstract
Addition of cyclic AMP to normal rabbit reticulocyte lysate brings about substantial increase in protein synthesis programmed by both nuclear and polysomal eukaryotic RNAs. The increase in synthesis is largely nonspecific and the in-vitro translation system is rendered viable for longer periods of time than in the absence of cyclic AMP. This stimulation of translation could be due to the inhibition of protein kinases that are initially activated by secondary structure of RNAs. However, the ability of cyclic AMP to further stimulate methyl mercury or heat denatured RNA indicates that additional mechanisms may be involved in the enhancement of translation