A differential proteome in tumors suppressed by an adenovirus‐based skin patch vaccine encoding human carcinoembryonic antigen

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Abstract
We created an anti‐tumor vaccine by using adenovirus as a vector which contains a cytomegalovirus early promoter‐directed human carcinoembryonic antigen gene (AdCMV‐hCEA). In an attempt to develop the skin patch vaccine, we epicutaneously vaccinated Balb/c mice with AdCMV‐hCEA. After nine weeks post‐immunization, vaccinated mice evoked a robust antibody titer to CEA and demonstrated the capability of suppressing in vivo growth of implanted murine mammay adenocarioma cell line (JC‐hCEA) tumor cells derived from a female Balb/c mouse. Proteomic analysis of the tumor masses in the non‐vaccinated naïve and vaccinated mice reveal that six proteins change their abundance in the tumor mass. The levels of adenylate kinase 1, β‐enolase, creatine kinase M chain, hemoglobin beta chain and prohibitin were statistically increased whereas the level of a creatine kinase fragment, which is undocumented, was decreased in the tumor of vaccinated mice. These proteins may provide a vital link between early‐stage tumor suppression and immune response of skin patch vaccination.