ANALYSIS OF DIVERSITY OF MURINE ANTIBODIES TO DEXTRAN B1355 .1. GENERATION OF A LARGE, PAUCI-CLONAL RESPONSE BY A BACTERIAL VACCINE

  • 1 January 1976
    • journal article
    • research article
    • Vol. 117  (2) , 569-575
Abstract
Mice immunized with a combination of dextran B1355 in adjuvant followed by 3 injections of 2 .times. 109 Escherichia coli B organisms produced an average of 14.5 mg/ml of anti-dextran antibodies. The stimulation effect of E. coli B was due to antigenic determinants cross-reactive with B1355 and not solely because of adjuvant properties of the organism. The anti-dextran antibodies were distributed among 7S and 19S components. Isoelectric focusing of the 7S antibodies showed several spectrotypes of antibody, most of which were shared by the majority of the individual sera. The limited spectrotypic heterogeneity of the 7S antibodies was supported by idiotypic studies. A heterologous, anti-idiotypic serum, rabbit anti-M104, was prepared which distinguished between 2 closely related myeloma proteins, M104 and J558, with specificity for .alpha.-(1 .fwdarw. 3) dextran. This antiserum demonstrated that some, but not all, of the 7S and 19S anti-dextran antibodies possessed variable region determinants cross-reactive with M104.

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