Filovirus-pseudotyped lentiviral vector can efficiently and stably transduce airway epithelia in vivo
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- 1 March 2001
- journal article
- research article
- Published by Springer Nature in Nature Biotechnology
- Vol. 19 (3) , 225-230
- https://doi.org/10.1038/85664
Abstract
Traditional gene therapy vectors have demonstrated limited utility for treatment of chronic lung diseases such as cystic fibrosis (CF). Herein we describe a vector based on a Filovirus envelope protein-pseudotyped HIV vector, which we chose after systematically evaluating multiple strategies. The vector efficiently transduces intact airway epithelium from the apical surface, as demonstrated in both in vitro and in vivo model systems. This shows the potential of pseudotyping in expanding the utility of lentiviral vectors. Pseudotyped lentiviral vectors may hold promise for the treatment of CF.Keywords
This publication has 38 references indexed in Scilit:
- Pseudotyped human lentiviral vector-mediated gene transfer to airway epithelia in vivoGene Therapy, 2000
- Feline immunodeficiency virus vectors persistently transduce nondividing airway epithelia and correct the cystic fibrosis defectJournal of Clinical Investigation, 1999
- Viral vector targetingCurrent Opinion in Biotechnology, 1999
- Lentiviral Vectors for Gene Therapy of Cystic FibrosisHuman Gene Therapy, 1997
- Cystic fibrosis.American Journal of Respiratory and Critical Care Medicine, 1996
- In vivo retroviral gene transfer into human bronchial epithelia of xenografts.Journal of Clinical Investigation, 1992
- Correction of the Apical Membrane Chloride Permeability Defect in Polarized Cystic Fibrosis Airway Epithelia Following Retroviral-Mediated Gene TransferHuman Gene Therapy, 1992
- Multipotent neural cell lines can engraft and participate in development of mouse cerebellumCell, 1992
- Risk Factors for Respiratory Syncytial Virus-associated Lower Respiratory Illnesses in the First Year of LifeAmerican Journal of Epidemiology, 1991
- Assembly and morphology of HIVAIDS, 1991