Genetic control of the glp system in Bacillus subtilis

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Abstract
In pleiotropic negative glycerol utilization mutants (GlpP1 mutants) of B. sutilis, glycerol kinase [EC 2.7.1.30] and sn-glycerol 3-phosphate (G3P) dehydrogenase [EC 1.1.99.5] are noninducible. GlpP1 mutants also fail to take up exogenous [14C]G3P. To study the regulation of the glp system in B. subtilis phenotypically, Glp+ revertants were isolated from GlpP1 mutants. Four classes of revertants were identified; phenotypically, wild type; R1 type, which contains an informational suppressor; R2 type, which produces G3P dehydrogenase constitutively; and R3 type, with a temperature-sensitive Glp phenotype producing G3P dehydrogenase constitutively at permissive temperature (32.degree. C). The properties of the revertants indicate that the glpP1 locus codes for a protein with a positive regulatory function.