Replication of four antigenic types of avian reovirus in subpopulations of chicken leukocytes

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Abstract
The replication of four antigenic types of avian reovirus in various subpopulations of avian leukocytes was investigated. Virus replication was detected in infected cells by immunofluorescence using a monoclonal antibody against a virion protein and by electron microscopy. All four types of reovirus replicated in cultured, adherent mononuclear cells of both bone marrow and peripheral blood origin causing lysis and fusion of the infected cells. Some evidence of strain variation in the capacity of avian reoviruses to replicate in these cells was detected. Avian reovirus did not replicate in heterophils or thrombocytes of peripheral blood origin or in bursa or thymus‐derived lymphocytes.