SOME BIOCHEMICAL CHARACTERISTICS OF VIBRIO FETUS AND OTHER RELATED VIBRIOS ISOLATED FROM ANIMALS

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Abstract
The reduction of triphenyl tetrazolium chloride in a Thunberg tube by resting cells of a typical strain of V. fetus in the presence of sodium lactate was chosen as a model system that was standardized with respect to the following optima: harvest time, pH of reduction reaction, concentration of triphenyl tetrazolium chloride, cell concentration and substrate concentration. Under these standard conditions other substrates were tested for their electron-donating capacity. Results showed a pattern of oxidative activity for one strain. The pattern for this strain was compared to the pattern of 26 other strains examined in a like manner. It was determined that the oxidative capacity of vibrios isolated from farm animals was quite similar regardless of whether they were isolated from different hosts, different anatomical locations, or different H2S-catalase types from the same host and same anatomical location. Only lactate, formate, pyruvate, [alpha]-ketoglutarate, and succinate out of 30 substrates tested served as electron donors. The growth in heart infusion broth of 11 out of 12 bovine catalase-positive strains of V. fetus was inhibited by the addition of 0.8% glycine while none of 5 similar ovine strains was inhibited.
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