Myosin V orientates the mitotic spindle in yeast

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Abstract
Coordination of spindle orientation with the axis of cell division is an essential process in all eukaryotes. In addition to ensuring accurate chromosomal segregation, proper spindle orientation also establishes differential cell fates and proper morphogenesis1. In both animal and yeast cells, this process is dependent on cytoplasmic microtubules interacting with the cortical actin-based cytoskeleton2,3,4,5, although the motive force was unknown. Here we show that yeast Myo2, a myosin V that translocates along polarized actin cables into the bud6, orientates the spindle early in the cell cycle by binding and polarizing the microtubule-associated protein Kar9 (refs 7,8,9). The tail domain of Myo2 that binds Kar9 also interacts with secretory vesicles12 and vacuolar elements13, making it a pivotal component of yeast cell polarization.