A fixation-decalcification procedure for bone biopsies

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Abstract
A procedure for the simultaneous fixation and decalcification of bone biopsies is described. Mild acidic fixatives (such as Bouin's fluid) are effective decalcifying agents when made calcium‐free by a continuous circulation through a column of anion‐binding ion exchange resin. The procedure has been found relatively fast, safe, economic and to provide excellent histology of biopsies containing cancellous bone.

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