A Simplified Method of Staining Thin Sections of Biological Material with Lead Hydroxide for Electron Microscopy

Open Access

1 April 1960

journal article
research article
Published by Rockefeller University Press in The Journal of cell biology

Vol. 7 (2) , 409-410
https://doi.org/10.1083/jcb.7.2.409

Abstract

It has been found that the contrast in thin sections prepared for electron microscopy, and stained with a saturated solution of lead acetate, may be increased by exposure to NH4OH vapor. Apparently, increased binding of lead by proteins occurs with increasing pH - thus lead acetate (pH 5.9) gives a light general electron stain, lead hydroxide (pH 8.15) a heavy general stain, while monobasic lead acetate (pH 7.0) selectively stains cell components known to contain nucleo-protein.

Keywords

MICROSCOPY, ELECTRON
STAINS AND STAINING

This publication has 1 reference indexed in Scilit:

Staining of Tissue Sections for Electron Microscopy with Heavy Metals
The Journal of cell biology, 1958