Fluorescence microscope to observe dynamical processes in monomolecular layers at the air/water interface
- 1 December 1984
- journal article
- Published by AIP Publishing in Review of Scientific Instruments
- Vol. 55 (12) , 1968-1972
- https://doi.org/10.1063/1.1137688
Abstract
An experimental setup is described to microscopically observe surfactant monolayers at the air/water interface. It basically consists of a film balance, incorporated into a fluorescence microscope, detecting the emission of dye probes. The apparatus is stable enough to observe dynamical processes over more than 5 h, with a spatial resolution of less than 2 μm and is sensitive enough to use probe concentrations as low as 0.1 mol%. Examples of surface textures appearing during lipid phase transitions demonstrate the good performance characteristics.This publication has 7 references indexed in Scilit:
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