Determination of glycerol in wine by amperometric flow injection analysis with an immobilized glycerol dehydrogenase reactor.

1 January 1991

journal article
Published by Oxford University Press (OUP) in Agricultural and Biological Chemistry

Vol. 55 (4) , 1055-1059
https://doi.org/10.1271/bbb1961.55.1055

Abstract

A glycerol sensor, in which glycerol dehydrogenase was immobilized on Amino-Cellulofine, was developed for the continuous determination of glycerol in wine. NADH produced by the enzyme reaction was monitored amperometrically with a platinum electrode. A sandwich method was used for sample injection as an effective mixing mode of NAD and sample. A typical calibration curve showed a slightly convex curve between response and glycerol concentration (0.1-2 HIM; 1.8 × 10^-8 -3.6× 10^-7mol). The relative standard deviation for ten measurements of 9×10^-8 mol glycerol was 0.5%. The proposed method was used for wine analysis and was compared with an F-kit (enzymatic, spectrophotometric) method.

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