Patterns of histone acetylation in Physarum polycephalum

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Abstract
Histone acetylation has previously been correlated with both chromosome replication and transcription. Both correlations were confirmed in the true slime mold P. polycephalum and quite a different pattern of acetate turnover was associated with replication compared with transcription. The pattern associated with replication involved turnover of acetate on all 4 core histones on species containing 1 or 2 acetates/molecule. This pattern was resolved from the transcription-associated pattern by 3 different procedures: detailed analysis of gels of histones pulse-labeled with acetate; the pattern of acetylation of histones pulse-labeled with [3H]lysine; and the pattern of acetylation of soluble histones. The pattern associated with transcription was restricted to histones H3 and H4 and occurred mostly on highly acetylated species. This pattern was resolved by analysis of gels of histones pulse-labeled with acetate; the pattern of histone acetylation in G2 phase of the cell cycle; and the pattern of histone acetylation in the presence of cycloheximide.