Quantitative Liquid Chromatography of Allopurinol and Oxypurinol in Human Plasma and Urine
- 1 September 1985
- journal article
- research article
- Published by Wolters Kluwer Health in Therapeutic Drug Monitoring
- Vol. 7 (3) , 324-328
- https://doi.org/10.1097/00007691-198507030-00017
Abstract
Summary: A high performance liquid chromatographic (HPLC) assay is described for allopurinol and oxypurinol determination in human plasma and urine, in the range expected during therapy. The procedure involves addition of trichloroacetic acid to samples, followed by centrifugation. The supernatant is then neutralized and analyzed by reversed-phase HPLC. Characteristics of the method are reported, and data are presented on its application to the pharmacokinetics studies. Separation is optimal with an octadecylsilane (ODS) stationary phase and a sodium acetate mobile phase adjusted to pH 7.2 for plasma and pH 5 for urine.Keywords
This publication has 5 references indexed in Scilit:
- High-performance liquid chromatography with polarographic and voltammetric anodic detection: Simultaneous determination of allopurinol, oxipurinol and uric acid in body fluidsJournal of Chromatography B: Biomedical Sciences and Applications, 1984
- Simultaneous liquid chromatography of 5-fluorouracil, uridine, hypoxanthine, xanthine, uric acid, allopurinol, and oxipurinol in plasma.Clinical Chemistry, 1980
- Quantitative high-performance liquid chromatography of nucleosides and bases in human plasmaJournal of Chromatography B: Biomedical Sciences and Applications, 1980
- The determination of allopurinol and oxipurinol in human plasma and urineJournal of Chromatography B: Biomedical Sciences and Applications, 1977
- APPARENT METABOLISM OF ALLOPURINOL BY BLOOD - PRELIMINARY-REPORT1977