VIROIMMUNOENZYMOASSAY FOR DETECTION OF ANTI-PENICILLOYL ANTIBODIES AND PENICILLOYL RESIDUES - COMPARISON OF RESULTS OBTAINED BY RADIOIMMUNOASSAY VIROIMMUNOASSAY AND ENZYMOIMMUNOASSAY

  • 1 January 1979
    • journal article
    • research article
    • Vol. C130  (3) , 407-418
Abstract
A new technique, viroimmunoenzymoassay, was described. The principle is the same as in classical viroimmunoassay, which uses a bacteriophage bound covalently to a hapten as tracer. Immuno-specific neutralization of these modified bacteriophages by hapten [rabbit] antiserum allows the detection and the testing of hapten antibodies or haptens with great sensitivity. The visualization of the bacterial lysis is estimated by measuring the amount of .beta.-galactosidase released into the medium by Escherichia coli BB bacteria. This is induced by isopropyl thiogalactoside or lactose. The method is applied to the assay of penicilloyl groups bound covalently with another molecule and its performance is compared with 3 other classical immunological methods: ratio-, enzymo- and viroimmunoassay.

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