Some Properties of Protease fromBacillusR-4 Which LysesRhizopusCell Wall
- 1 October 1976
- journal article
- research article
- Published by Oxford University Press (OUP) in Agricultural and Biological Chemistry
- Vol. 40 (10) , 1937-1943
- https://doi.org/10.1080/00021369.1976.10862343
Abstract
A strain of Bacillus sp. (Bacillus R-4) produces a protease and a chitosanase which have the ability of lysing Rhizopus cell wall. Some enzymatic properties of the protease purified to a homogeneous state were examined. The molecular weight of the enzyme was estimated as 19,000 and the isoelectric point as pH 8.65. The protease appeared to have a relative wide range of substrate specificity, hydrolyzing various proteins, such as gelatin, hemoglobin and protamine, and synthetic peptides, such as Z-Gly-Try-NH2, Z-Gly-Ala-NH2, Z-Ala-Leu-NH2 and Z-Gly-Leu-NH2. The activity lost by EDTA and by Hg2+ was restored by Zn2+ and reduced glutathione, respectively.This publication has 2 references indexed in Scilit:
- Comparison of the specificities of various neutral proteinases from microorganismsArchives of Biochemistry and Biophysics, 1968
- Estimation of the molecular weights of proteins by Sephadex gel-filtrationBiochemical Journal, 1964