99Tcmexametazime-labelled leucocytes

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Abstract
We have investigated the effect of volume and concentration of exametazime on the labelling efficiency of 99Tcm-exametazime-labelled leucocytes. The first study examined the effect of varying the volume of exametazime solution whilst the concentration remained constant. A vial of Ceretec (Amersham Ltd plc) was reconstituted with sodium chloride injection BP. Aliquots of 0.25–2.0 ml were removed, added to sodium pertechnetate injection BP and incubated with 2 × 107-5 × 107 leucocytes from 25 ml blood. The labelling efficiency decreased from 65 ±10% s.d. (0.25 ml) to 45 ± 8% (2.0 ml) (n = 4). In a second study different concentrations of exametazime solution were used whilst the volume remained constant. A vial of Ceretec was reconstituted with sodium chloride injection BP. Aliquots of 25–200μg were removed, made up to a fixed volume of 0.6 ml, added to pertechnetate and incubated with the plug of leucocytes as before. The labelling efficiency decreased as the concentration of ligand decreased. Thus for 200 μ-g/0.6 ml the labelling efficiency was 64 ± 5% and for 25μg/0.6 ml the labelling efficiency was 43 ± 18% (n = 4). Clinical studies were performed using 50 ml blood from patients with a wide range of inflammatory disorders including inflammatory bowel disease, abdominal abscess and vascu-litis. The concentration of ligand used was 83 μg/0.25 ml. The labelling efficiency was found to be 82 ± 77% (n = 36).

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