Manganese Encrustation of Zygospores of a Chlamydomonas (Chlorophyta: Volvocales)

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Abstract
In media containing normal trace-element supplements, but not in manganese-deficient media, zygospores of a new species of Chlamydomonas (isolated from soil) become encrusted with a dark brown mineral coating. Staining with benzidine indicates that the encrustation is rich in manganese. This has been confirmed by x-ray analysis in combination with a scanning electron microscope.