Polymerase chain reaction‐based detection of MN blood group‐specific sequences in the human genome
- 28 February 1993
- journal article
- research article
- Published by Wiley in Transfusion
- Vol. 33 (2) , 119-124
- https://doi.org/10.1046/j.1537-2995.1993.33293158042.x
Abstract
The MN blood group antigens have traditionally been detected by serotyping; however, development of a DNA-based method offers flexibility in the determination of this highly polymorphic system. Genotyping the MN blood group antigens was performed by polymerase chain reaction amplification of the specific alleles (PASA) in the human genome. In separate paired reactions, M or N allele-specific oligonucleotide primers were amplified with a common distal primer. Only in the presence of the homologous template was a 781-base pair polymerase chain reaction amplification product visible after agarose gel electrophoresis and ethidium bromide staining. This method of genotyping could be performed using either 1 μg of extracted DNA or 0.5 microL of whole blood, and the results showed 100-percent correlation with those obtained by serotyping. PASA-based genotyping of MN blood group antigens, which requires a small amount of starting material, has application in linkage and population studies and in forensic medicine.Keywords
This publication has 18 references indexed in Scilit:
- Application of an allele-specific polymerase chain reaction to the direct determination of ABO blood group genotypesGenomics, 1992
- Promoter sequence and chromosomal organization of the genes encoding glycophorins A, B and EGene, 1990
- Direct PCR from whole blood, without DNA extractionNucleic Acids Research, 1990
- A novel gene member of the human glycophorin A and B gene familyEuropean Journal of Biochemistry, 1990
- Molecular Genetics of Human Erythrocyte Sialoglycoproteins Glycophorins A, B, C, and DPublished by Springer Nature ,1990
- Structural organization of glycophorin A and B genes: glycophorin B gene evolved by homologous recombination at Alu repeat sequences.Proceedings of the National Academy of Sciences, 1989
- Avoiding false positives with PCRNature, 1989
- Allele-specific enzymatic amplification of beta-globin genomic DNA for diagnosis of sickle cell anemia.Proceedings of the National Academy of Sciences, 1989
- Molecular cloning of a human glycophorin B cDNA: nucleotide sequence and genomic relationship to glycophorin A.Proceedings of the National Academy of Sciences, 1987
- Blot hybridisation analysis of genomic DNA.Journal of Medical Genetics, 1984