Pitfalls in the assay of carboxymethylcellulase activity
- 31 January 1983
- journal article
- research article
- Published by Wiley in Biotechnology & Bioengineering
- Vol. 25 (2) , 377-385
- https://doi.org/10.1002/bit.260250207
Abstract
A purified endocellulase from Sclerotium rolfsii and a crude cellulase preparation from Trichoderma reesei are used to illustrate several pitfalls associated with the assay of carboxymethylcellulase activity and the subsequent attainment of linear enzyme dilution curves. It is shown that the nature of both the enzymes and the substrate make the assay unsuitable for use in the calculation of enzyme recovery and purity.This publication has 14 references indexed in Scilit:
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