Mechanism of stimulation of in vitro protein synthesis by some copolymers of styrene, vinyluracil, and vinyladenine with maleic acid and acrylic acid
- 1 August 1976
- journal article
- research article
- Published by American Chemical Society (ACS) in Biochemistry
- Vol. 15 (16) , 3536-3540
- https://doi.org/10.1021/bi00661a022
Abstract
Copolymers of vinyl bases with acrylic acid and styrene or 1-vinyluracil with maleic acid stimulated in vitro polyphenylalanine synthesis, using a system extracted from Escherichia coli MRE600. Poly(styrene-maleic acid) inhibited ribosomal bound RNase. Poly(1-vinyluracil,maleic acid), poly(1-vinyluracil,acrylic acid) and poly(9-vinyladenine,acrylic acid) were not inhibitors of the ribosome bound RNase. The potent (up to 5-fold) stimulation by these 3 polymers is due to the action of the polymers to interfere with ribosomal bound inhibitory protein. A protein, removed by washing ribosomes with 1 M ammonium chloride, characterized by M.J. MILLER. A. Niveleau and A.J. Wahba, was described as a potent inhibitor of in vitro poly(U)-coded protein synthesis using extracts of E. coli MRE600.Keywords
This publication has 1 reference indexed in Scilit:
- Purification and Properties of Ribonuclease II from Escherichia coliJournal of Biological Chemistry, 1964