Formation of internucleotide 3′-5′ phosphoramidate links by direct coupling of phosphoryl and amino groups
Open Access
- 1 January 1975
- journal article
- Published by Oxford University Press (OUP) in Nucleic Acids Research
- Vol. 2 (7) , 1123-1128
- https://doi.org/10.1093/nar/2.7.1123
Abstract
The stepwise synthesis of oligomers derived from 5′-amino-5′-deoxythymidine 3′-phosphate and 5′-amino-5′-deoxy-3′-O-mono-P-methoxytritylthymidine is described. The internucleotide phosphoramidate links were formed by condensation of nucleoside 3′-phosphates with aminonucleosides by means of triphenylphosphine and dipyridyl disulfide.Keywords
This publication has 5 references indexed in Scilit:
- Use of phosphorus oxychloride in synthesizing nucleotides and ollgonucleotidesNucleic Acids Research, 1974
- Use of trityl- and .alpha.-naphthylcarbamoylcellulose derivatives in oligonucleotide synthesisBiochemistry, 1973
- Synthesis of oligothymidylates and nucleoside cyclic phosphates by oxidation-reduction condensationJournal of the American Chemical Society, 1972
- Nucleotide chemistry. XVII. Enzymic synthesis of polydeoxyribonucleotides possessing internucleotide phosphoramidate bondsJournal of the American Chemical Society, 1972
- Nucleotide chemistry. XVI. Phosporamidate analogs of oligonucleotidesThe Journal of Organic Chemistry, 1970