GELATIN PARTICLE AGGLUTINATION ASSAY FOR HIV ANTIBODIES - A RAPID, ECONOMICAL MODIFICATION WITH INCREASED SENSITIVITY

  • 1 April 1989
    • journal article
    • research article
    • Vol. 46  (2) , 135-140
Abstract
Modification of a commercial gelatin particle agglutination assay for anti-HIV reduces the test time to 30 min, increases the sensitivity sevenfold without any prozoning, and maintains specificity while cutting the cost of the test by 90%. The modification involves a tenfold dilution of the gelatin particles, which are added to a dilution of test serum in a ''V'' well standard microplate. After incubation, plates are centrifuged briefly and allowed to stand at an inclination of 70.degree. until positive and negative reactions are clearly distinguishable within approximately 15 min.

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