Isolation of Patient-Related Antigens from Allergen Extracts by Means of Protein A-Sepharose-Patient IgG1,2,4Bio-Affinity Chromatography

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Abstract
A method is described for bio-affinity purification of patient-specific IgG reactive antigens by using protein A-sepharose allergic patient serum and the corresponding antigen/allergen extract. The optimal conditions for each step were determined. The non-specific binding of serum proteins and antigens can be kept at very low levels by a high ionic strength. The specificity of the reaction was shown by biospecific elution of 125I-labeled antigens by non-labeled antigens and also by comparison of the antigen bound by sera from hyposensitized patients with that from non-atopic individuals. The isolated antigen-IgG-immune complexes could be used for a specific and active immunotherapy.