Role of Molybdenum as a Constituent of Nitrate Reductase from Soybean Leaves.

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Abstract
Molybdenum, already established as the metal component of Neurospora nitrate reductase, has also been identified as the metal of nitrate reductase of soybean leaves. This is supported by the proportionality between Mo content and specific enzyme activity, as well as the restoration of dialyzed enzyme specifically by M0O3, or Na2MoO4. Other micronutrient elements including Fe, Cu, Mn, Co, Ni, W. V. and B, respectively, were without effect. It has also been shown that during the enzymatic transfer of electrons from DPNH to nitrate, flavin and Mo function as electron carriers in the following sequence: DPNH[long dash][long dash]> FAD (or FMN)[long dash][long dash]-> Mo[long dash]> NO3 FMNH2 or reduced molybdate (without added flavin) can replace reduced pyridine nucleotides in the enzymatic reduction of nitrate to nitrite.