Effect of Neem Seed Extract upon the Gypsy Moth (Lepidoptera: Lymantriidae) and Its Nuclear Polyhedrosis Virus

Abstract
Neem seed extract inhibited growth and development of gypsy moth, Lymantria disbar (L.), larvae. Untreated control larvae increased their weight by ≍40-fold by day 14, whereas insects treated with 0.10% neem and 1.00% neem weighed ≍30% and ≍4%, respectively, of the average weight of the untreated larvae. By day 14, 99% of the controls were in the fifth stage and 1% were prepupae. After treatment with 0.10% neem almost one-third of those larvae were still in the fourth stage, whereas larvae treated with 1.00% neem were still in the second and third stages. Neem extracts had little, if any, effect upon viral activity, as measured by LC50s. The addition of neem extract to the gypsy moth nuclear polyhedrosis virus, however, resulted in faster virus-caused mortality.

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