Cell Cycle Arrest in Archaea by the Hypusination Inhibitor N 1 -Guanyl-1,7-Diaminoheptane
Open Access
- 15 February 2000
- journal article
- Published by American Society for Microbiology in Journal of Bacteriology
- Vol. 182 (4) , 1158-1161
- https://doi.org/10.1128/jb.182.4.1158-1161.2000
Abstract
Hypusination is an essential posttranslational modification unique to archaeal and eukaryotic protein synthesis initiation factor 5A (aIF5A and eIF5A, respectively). We have investigated the effect of the efficient hypusination inhibitor N 1 -guanyl-1,7-diaminoheptane (GC 7 ) on four archaeal and one bacterial species. We found that (i) archaea are sensitive to GC 7 , whereas the bacterium Escherichia coli is not, (ii) GC 7 causes rapid and reversible arrest of growth of the archaeon Sulfolobus acidocaldarius , and (iii) the growth arrest is accompanied by a specific reversible arrest of the cell cycle prior to cell division. Our findings establish a link between hypusination and sustained growth of archaea and thereby provide the framework to study molecular details of archaeal cell cycle in connection with in vivo functions of hypusine and of aIF5A and eIF5A.Keywords
This publication has 16 references indexed in Scilit:
- Universally conserved translation initiation factorsProceedings of the National Academy of Sciences, 1998
- Cell cycle characteristics of thermophilic archaeaJournal of Bacteriology, 1997
- Hypusine Is Essential for Eukaryotic Cell ProliferationNeurosignals, 1997
- Effects of inhibitors of deoxyhypusine synthase on the differentiation of mouse neuroblastoma and erythroleukemia cellsCancer Letters, 1996
- Effects of N1-guanyl-1,7-diaminoheptane, an inhibitor of deoxyhypusine synthase, on the growth of tumorigenic cell lines in cultureBiochimica et Biophysica Acta (BBA) - Molecular Cell Research, 1996
- Diamine and Triamine Analogs and Derivatives as Inhibitors of Deoxyhypusine Synthase: Synthesis and Biological ActivityJournal of Medicinal Chemistry, 1995
- Effect of initiation factor eIF-5A depletion on protein synthesis and proliferation of Saccharomyces cerevisiae.Journal of Biological Chemistry, 1994
- Features of the spermidine-binding site of deoxyhypusine synthase as derived from inhibition studies. Effective inhibition by bis- and mono-guanylated diamines and polyaminesJournal of Biological Chemistry, 1993
- The Unique Posttranslational Modification Leading to Deoxyhypusine or Hypusine is a General Feature of the Archaebacterial KingdomSystematic and Applied Microbiology, 1990
- Archaebacterial Protein Contains Hypusine, a Unique Amino Acid Characteristic for Eukaryotic Translation Initiation Factor 4DSystematic and Applied Microbiology, 1989